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immunofluorescence marker cd68  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology immunofluorescence marker cd68
    Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and <t>CD68-positive</t> macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained <t>CD68-positive</t> atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).
    Immunofluorescence Marker Cd68, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence marker cd68/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    immunofluorescence marker cd68 - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from in situ spatial whole transcriptomic analysis"

    Article Title: CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from in situ spatial whole transcriptomic analysis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1475051

    Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and CD68-positive macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained CD68-positive atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).
    Figure Legend Snippet: Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and CD68-positive macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained CD68-positive atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).

    Techniques Used: Marker, Staining, Selection, Control

    Differentially expressed genes (DEGs) in CD68-positive macrophages from spatial whole transcriptomic analysis [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Volcano plot displaying highly expressed DEGs in CD68-positive macrophages based on the degree of CRP deposition in AAA, using log 10 fold changes. (B) Clustered heatmap of the selected ROIs showing whole transcriptomic expression fold changes (average gene expression, z-score) in relation to CRP levels in AAA. Approximately 1,100 genes were differentially expressed according to the CRP deposition level. (C) Key DEGs between the high-CRP and low-CRP groups from (A) . The asterisk symbol (*) indicates statistical significance at p < 0.05.
    Figure Legend Snippet: Differentially expressed genes (DEGs) in CD68-positive macrophages from spatial whole transcriptomic analysis [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Volcano plot displaying highly expressed DEGs in CD68-positive macrophages based on the degree of CRP deposition in AAA, using log 10 fold changes. (B) Clustered heatmap of the selected ROIs showing whole transcriptomic expression fold changes (average gene expression, z-score) in relation to CRP levels in AAA. Approximately 1,100 genes were differentially expressed according to the CRP deposition level. (C) Key DEGs between the high-CRP and low-CRP groups from (A) . The asterisk symbol (*) indicates statistical significance at p < 0.05.

    Techniques Used: Expressing, Gene Expression

    Functional enrichment and protein–protein interaction analysis of GO terms related to CRP deposition in AAA tissues: in CD68-positive macrophages [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Scatter plot from REVIGO illustrating cluster representatives after redundancy reduction in a two-dimensional space. Multidimensional scaling was applied to a matrix of the 34 GO terms’ semantic similarities. Notable GO terms identified include inflammatory response and blood vessel diameter maintenance. The bubble size indicates the frequency of the GO terms in the underlying GOA database, with color shading representing user-supplied p-values (legend in the lower right corner). (B) Potential enriched GO pathways in CD68+/CRP (high/low) regions. A total of 34 statistically significant biological process GO terms were observed. Further analysis highlighted representative GO terms such as inflammatory responses and MAPK cascade. Protein–protein interaction analysis using the STRING program with high confidence revealed that “ STAT3 ” connects two other clusters associated with apoptosis and immune responses within the inflammation cluster. (C) GO analysis results of genes associated with “blood vessel diameter maintenance,” and “inflammation response.” DEG, differentially expressed gene; Down, downregulated; Up, upregulated; AAA–high CRP, abdominal aortic aneurysm with high CRP; AAA–low CRP, abdominal aortic aneurysm with low CRP. The asterisk symbol (*) indicates statistical significance at p < 0.05.
    Figure Legend Snippet: Functional enrichment and protein–protein interaction analysis of GO terms related to CRP deposition in AAA tissues: in CD68-positive macrophages [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Scatter plot from REVIGO illustrating cluster representatives after redundancy reduction in a two-dimensional space. Multidimensional scaling was applied to a matrix of the 34 GO terms’ semantic similarities. Notable GO terms identified include inflammatory response and blood vessel diameter maintenance. The bubble size indicates the frequency of the GO terms in the underlying GOA database, with color shading representing user-supplied p-values (legend in the lower right corner). (B) Potential enriched GO pathways in CD68+/CRP (high/low) regions. A total of 34 statistically significant biological process GO terms were observed. Further analysis highlighted representative GO terms such as inflammatory responses and MAPK cascade. Protein–protein interaction analysis using the STRING program with high confidence revealed that “ STAT3 ” connects two other clusters associated with apoptosis and immune responses within the inflammation cluster. (C) GO analysis results of genes associated with “blood vessel diameter maintenance,” and “inflammation response.” DEG, differentially expressed gene; Down, downregulated; Up, upregulated; AAA–high CRP, abdominal aortic aneurysm with high CRP; AAA–low CRP, abdominal aortic aneurysm with low CRP. The asterisk symbol (*) indicates statistical significance at p < 0.05.

    Techniques Used: Functional Assay



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    Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and <t>CD68-positive</t> macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained <t>CD68-positive</t> atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).
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    Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and <t>CD68-positive</t> macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained <t>CD68-positive</t> atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).
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    Image Search Results


    Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and CD68-positive macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained CD68-positive atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).

    Journal: Frontiers in Immunology

    Article Title: CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from in situ spatial whole transcriptomic analysis

    doi: 10.3389/fimmu.2024.1475051

    Figure Lengend Snippet: Morphologic marker staining and area of illumination selection in aortic tissue analysis. (A) Whole TMA slide scan view images for a normal aorta, AAA with low CRP, and AAA with high CRP. Each TMA section was prepared by creating 3-mm-diameter cores from the aortic wall, focusing on areas in contact with atheroma if present [AAA–low-CRP (n = 3), AAA–high-CRP (n = 7), and control group (n = 3)]. (B) Representative sections of a normal aorta, AAA–low CRP, and AAA–high CRP. The normal aorta primarily consists of αSMA-positive vascular smooth muscle cells with minimal inflammatory cell presence. In AAA–low CRP, the αSMA-positive vascular wall is thinner with fibrotic changes (white arrow), and CD68-positive macrophage infiltration is observed between fibrotic regions (red arrow). In AAA–high CRP, an extensively stained CD68-positive atherosclerotic plaque (magenta arrow) accumulates on a significantly thinned αSMA-positive vascular smooth muscle cell layer (yellow arrow). (C) Representative image of AOI segmentation using IF morphologic markers. The atheromatous plaque and vascular smooth muscle interface were stained simultaneously with CD45, αSMA, and CD68 markers. Vascular smooth muscle segmentation is highlighted (yellow arrow).

    Article Snippet: To perform transcriptomic analysis of specific cell types in aortic aneurysm, we used four fluorochromes (AF488, AF532, AF594, and AF647) to label the following immunofluorescence (IF) markers: αSMA (#ab202368, Abcam, Cambridge, UK), CD45 (#NBP2-34528AF532, Novus, CO, USA), and CD68 (#sc-20060AF647, Santa Cruz, TX, USA) ( ).

    Techniques: Marker, Staining, Selection, Control

    Differentially expressed genes (DEGs) in CD68-positive macrophages from spatial whole transcriptomic analysis [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Volcano plot displaying highly expressed DEGs in CD68-positive macrophages based on the degree of CRP deposition in AAA, using log 10 fold changes. (B) Clustered heatmap of the selected ROIs showing whole transcriptomic expression fold changes (average gene expression, z-score) in relation to CRP levels in AAA. Approximately 1,100 genes were differentially expressed according to the CRP deposition level. (C) Key DEGs between the high-CRP and low-CRP groups from (A) . The asterisk symbol (*) indicates statistical significance at p < 0.05.

    Journal: Frontiers in Immunology

    Article Title: CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from in situ spatial whole transcriptomic analysis

    doi: 10.3389/fimmu.2024.1475051

    Figure Lengend Snippet: Differentially expressed genes (DEGs) in CD68-positive macrophages from spatial whole transcriptomic analysis [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Volcano plot displaying highly expressed DEGs in CD68-positive macrophages based on the degree of CRP deposition in AAA, using log 10 fold changes. (B) Clustered heatmap of the selected ROIs showing whole transcriptomic expression fold changes (average gene expression, z-score) in relation to CRP levels in AAA. Approximately 1,100 genes were differentially expressed according to the CRP deposition level. (C) Key DEGs between the high-CRP and low-CRP groups from (A) . The asterisk symbol (*) indicates statistical significance at p < 0.05.

    Article Snippet: To perform transcriptomic analysis of specific cell types in aortic aneurysm, we used four fluorochromes (AF488, AF532, AF594, and AF647) to label the following immunofluorescence (IF) markers: αSMA (#ab202368, Abcam, Cambridge, UK), CD45 (#NBP2-34528AF532, Novus, CO, USA), and CD68 (#sc-20060AF647, Santa Cruz, TX, USA) ( ).

    Techniques: Expressing, Gene Expression

    Functional enrichment and protein–protein interaction analysis of GO terms related to CRP deposition in AAA tissues: in CD68-positive macrophages [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Scatter plot from REVIGO illustrating cluster representatives after redundancy reduction in a two-dimensional space. Multidimensional scaling was applied to a matrix of the 34 GO terms’ semantic similarities. Notable GO terms identified include inflammatory response and blood vessel diameter maintenance. The bubble size indicates the frequency of the GO terms in the underlying GOA database, with color shading representing user-supplied p-values (legend in the lower right corner). (B) Potential enriched GO pathways in CD68+/CRP (high/low) regions. A total of 34 statistically significant biological process GO terms were observed. Further analysis highlighted representative GO terms such as inflammatory responses and MAPK cascade. Protein–protein interaction analysis using the STRING program with high confidence revealed that “ STAT3 ” connects two other clusters associated with apoptosis and immune responses within the inflammation cluster. (C) GO analysis results of genes associated with “blood vessel diameter maintenance,” and “inflammation response.” DEG, differentially expressed gene; Down, downregulated; Up, upregulated; AAA–high CRP, abdominal aortic aneurysm with high CRP; AAA–low CRP, abdominal aortic aneurysm with low CRP. The asterisk symbol (*) indicates statistical significance at p < 0.05.

    Journal: Frontiers in Immunology

    Article Title: CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from in situ spatial whole transcriptomic analysis

    doi: 10.3389/fimmu.2024.1475051

    Figure Lengend Snippet: Functional enrichment and protein–protein interaction analysis of GO terms related to CRP deposition in AAA tissues: in CD68-positive macrophages [AAA–high CRP (n = 7) versus AAA–low CRP (n = 3)]. (A) Scatter plot from REVIGO illustrating cluster representatives after redundancy reduction in a two-dimensional space. Multidimensional scaling was applied to a matrix of the 34 GO terms’ semantic similarities. Notable GO terms identified include inflammatory response and blood vessel diameter maintenance. The bubble size indicates the frequency of the GO terms in the underlying GOA database, with color shading representing user-supplied p-values (legend in the lower right corner). (B) Potential enriched GO pathways in CD68+/CRP (high/low) regions. A total of 34 statistically significant biological process GO terms were observed. Further analysis highlighted representative GO terms such as inflammatory responses and MAPK cascade. Protein–protein interaction analysis using the STRING program with high confidence revealed that “ STAT3 ” connects two other clusters associated with apoptosis and immune responses within the inflammation cluster. (C) GO analysis results of genes associated with “blood vessel diameter maintenance,” and “inflammation response.” DEG, differentially expressed gene; Down, downregulated; Up, upregulated; AAA–high CRP, abdominal aortic aneurysm with high CRP; AAA–low CRP, abdominal aortic aneurysm with low CRP. The asterisk symbol (*) indicates statistical significance at p < 0.05.

    Article Snippet: To perform transcriptomic analysis of specific cell types in aortic aneurysm, we used four fluorochromes (AF488, AF532, AF594, and AF647) to label the following immunofluorescence (IF) markers: αSMA (#ab202368, Abcam, Cambridge, UK), CD45 (#NBP2-34528AF532, Novus, CO, USA), and CD68 (#sc-20060AF647, Santa Cruz, TX, USA) ( ).

    Techniques: Functional Assay